High efficiency, homology-directed genome editing in C. elegans using CRISPR/Cas9 ribonucleoprotein complexes
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High efficiency, homology-directed genome editing in C. elegans using CRISPR/Cas9 ribonucleoprotein complexes Alexandre Paix, Andrew Folkmann, Dominique Rasoloson, Geraldine Seydoux HHMI and Dept of Molecular Biology and Genetics, Johns Hopkins University, School of Medicine, Baltimore MD 21205, USA Running title: Genome editing in C. elegans using CRISPR/Cas9 RNP complexes
منابع مشابه
High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes
Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease Cas9 has become a popular method for genome editing in several organisms. Most HDR protocols rely on plasmid-based expression of Cas9 and the gene-specific guide RNAs. Here we report that direct injection of in vitro-assembled Cas9-CRISPR RNA (crRNA) trans-activating crRNA (tracrRNA) ribonucleoprotein complexes into...
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تاریخ انتشار 2015